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Biology Biotechnology and Its Application Skill Builder Single Correct MCQ
Published on: August 21, 2026

Mark the given statements True (T) or False (F).

(i) Green Revolution succeeded in quadrupling the food supply.

(ii) Organisms (plants, animal, bacteria) whose genes have been altered by manipulation are called Genetically Modified Organism (GMO).

(iii) Bt toxin is produced by a bacterium called Bacillus thuringiensis.

(iv) Some strains of Bacillus thurinigiensis produce protein that kill certain insects such as lepidopterans, Coleopterans and dipterans.

(v) Once Bt toxins is ingested by insect, the protoxins is converted into active form due to alkaline pH of gut and binds to hindgut epithelial cells and create pores.

(vi) The Bt toxin is coded by a gene named cry.

(vii) The Bt toxin protein encoded by genes cryIAc and cryIAb controls cotton bollworms, and that of cryIIAb controls corn borer.

(viii) A nematode Meloidogyne incognita infects the roots of tobacco plants and causes a great reduction in yield.

(ix) RNA interference method involves silenching of specific mRNA due to complementary ssRNA molecule that binds to and prevents translation of the mRNA.

(x) Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant tobacco.

Choose the correct option given below:

A
T T T F T F F T F T
B
F T T T F T F T T F
C
F T T T F T F T F T
D
T F F T T F F T T T

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Text Solution

Verified by Experts
The correct answer is:
C

(i) Green Revolution succeeded in quadrupling the food supply. F

Green Revolution greatly increased food production, but it did not quadruple the food supply.
(ii) GMOs are organisms whose genes have been altered by manipulation. → T

This is the definition of Genetically Modified Organisms (GMOs).
(iii) Bt toxin is produced by Bacillus thuringiensis. → T

The bacterium Bacillus thuringiensis (Bt) produces insecticidal proteins.
(iv) Some Bt strains kill Lepidopterans, Coleopterans and Dipterans. → T

Different Bt strains produce toxins effective against these insect groups.
(v) Bt toxin binds to hindgut epithelial cells and creates pores. → F

The activated toxin binds to midgut epithelial cells, not hindgut cells.
(vi) Bt toxin is coded by cry genes. → T

The insecticidal proteins are encoded by cry genes.
(vii) crylAc and crylAb control cotton bollworms, and cryllAb controls corn borer. → F

NCERT states crylAb is effective against corn borer, while crylAc is effective against cotton bollworm.
(viii) Meloidogyne incognita infects tobacco roots and reduces yield. → T

This root-knot nematode causes significant crop loss.
(ix) RNA interference involves complementary ssRNA. → F

RNAi involves double-stranded RNA (dsRNA), which silences the target mRNA.
(x) Agrobacterium vectors were used to introduce nematode-specific genes into tobacco. → T

This was done to produce dsRNA and confer resistance against the nematode.

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